Determination of Caffeine and some other Drugs in Cappuccino, Nescafe, Cacao, Coffee samples collected from some Libyan Markets using High Performance Liquid Chromatography (RP-HPLC)

This study aims to determine the caffeine and detection of some other drugs (which has commonly spread caused suicides in recent years El-Bieda city - Libya) cappuccino, nescafe, cacao coffee samples using Reversed Phase High Performance Liquid Chromatography (RP-HPLC). In presented study, seventeen were collected from Libyan markets. A rapid, simple reliable extraction method is developed validated for determination under dichloromethan, n-hexane methanol as extracting solvents. The over a wide linear range 2 – 10 µg/ml with correlation coefficients being consistently greater than 0.999. minimum level was observed Nesquik (Cacao), Nestle Italiana S.P.A sample (2.9129 µg/ml), while Nestla (Nescafe), Asbania showed highest content (598.5315 µg/ml). RP-HPLC measurements indicate that results concentrations are 2.9129 598.5315 an average 221.8630 µg/ml. by HPLC methods free drugs: (olanzapine, diazepam alprazolam). all analyzed this within allowable limits set US Food Drugs Administration documented values.